RE: [HAPS-L] Serum collection

["Harbut, Chester" <charbut@xxxxxxxxxxxx>]

Thank you everyone for your responses.  I simply collected the blood in an untreated (red cap) tube
and spun it down.  Worked perfectly.  

Alan, I used serum rather than plasma because I thought the clotting proteins would interfere with
the measurement of serum proteins as indicated by the protocol of this test.  I've seen similar 
suggestions in that vein ( no pun intended) when I Googled serum collection.

Anyways, the lab (spectrophotmetric measurement of serum proteins) was a success.  Kids learned 
a lot and so did their teacher.  That's what it's all about, no?!

Thanks again,

Chet Harbut
Cerritos Community College
11110 E. Alondra Blvd
Norwalk, CA 90650
562-860-2451 x-2684

Remember: Don't believe everything you think!!


-----Original Message-----
From: HAPS-L-owner@xxxxxxxxxxx on behalf of James S. Miller
Sent: Wed 1/18/2006 7:08 AM
To: HAPS-L@xxxxxxxxxxx
Subject: Re: [HAPS-L] Serum collection

        If you put it into a glass tube without anticoagulant, it should 
start to coagulate pretty quickly.  I haven't done it in mammalian blood 
for a long time, but I think in a minute or two it will start.  I'm not 
sure how long it takes to finish.
        A couple of years ago we were collecting frog blood using EDTA as 
an anticoagulant.  It turns out that EDTA doesn't work for amphibian 
blood.  The blood was coagulating almost as soon as it hit the glass tube - 
there was definite coagulation within 5-10 sec., and the process was 
complete in a few minutes.

At 05:54 AM 1/18/2006 -0800, you wrote:
> Sorry to piggback this on Alan's email but I often have trouble posting a 
> new topic.  Anyway, can anyone tell me the fastest way to
> collect SERUM from a blood sample (mine).  I know if you centrifuge a 
> blood sample in a heparinized tube that you'll get plasma.  And
> that if you let the blood just sit in an unheparinized tube you'll get 
> serum.  But what if you don't have a lot of time to wait for all
> that blood to clot and settle out?  Are there faster methods?
>
> Thanks in advance,
>
> Chet Harbut
>
> -----Original Message-----
> From: HAPS-L-owner@xxxxxxxxxxx on behalf of Alan Magid
> Sent: Wed 1/18/2006 12:30 AM
> To: IPaul@xxxxxxxxxx
> Cc: HAPS
> Subject: [HAPS-L] Nice link!
>
> Izak-
>
> The knee link is a great example of good computer-based learning. Thanks for
> sending it in.
>
> [We all should be on the prowl for other helpful online learning sites and
> send them onto to the list.]
>
> I sent it along to my step-daughter, now a first-year medical student.
>
> -Alan


**************************************************************************
James S. Miller, Ph.D.                        Phone: 574-535-7308
Chair & Professor of Biology
Goshen College                                 E-mail: JamesSM@xxxxxxxxxx
Goshen IN 46526-4795 USA                    Web: http://www.goshen.edu
************************************************************************** 

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